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名称 Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual
索引号 Q949.32-62/E20021/2005
分类号 Q949.32-62/E20021
Q949.32(真菌门)
Q949.3(菌类)
Q949.(孢子植物-植物)
Q949(植物分类学(系统植物学))
Q94(植物学)
Q(生物科学)
作者 Amberg、 David C.、Amberg、 David C.、Burke、 Dan、 1954-、Strathern、 Jeffrey N.、Burke、 Dan、 1954- Methods in yeast genetics.、Cold Spring Harbor Laboratory.、
出版社 Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2005.
ISBN
页数 xvii, 230 p. : ill. ; 28 cm.
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注解 Rev. ed. of: Methods in yeast genetics / Dan Burke, Dean Dawson, Tim Stearns, 2000 ed. 2000. 附註 Includes bibliographical references. 內容 Looking at yeast cells -- Isolation and characterization of auxotrophic, temperature-sensitive, and osmotic-sensitive mutants -- Meiotic mapping -- Mitotic recombination and random spore analysis -- Transformation of yeast -- Synthetic lethal mutants -- Gene replacement -- Isolation of ras2 suppressors -- Manipulating cell types -- Isolating mutants by insertional mutagenesis -- Isolation of separation of function mutants by two-hybrid differential interaction screening -- High-efficiency transformation of yeast -- "Quick and dirty" plasmid transformation of yeast colonies -- Yeast DNA isolations -- Yeast protein extracts -- TAP purification -- Yeast RNA isolations -- Hydroxylamine mutagenesis of plasmid DNA -- Assay of beta-galactosidase in yeast -- Plate assay for carboxypeptidase Y -- Random spore analysis -- Yeast vital stains -- Yeast immunofluorescence -- Actin staining in fixed cells -- PCR-mediated gene disruption -- Yeast colony PCR -- Measuring yeast cell density by spectrophotometry -- Cell synchrony -- Chromatin immunoprecipitation -- Flow cytometry of yeast DNA -- Logarithmic growth -- EMS mutagenesis -- Tetrad dissection -- Making a tetrad dissection needle -- Picking zygotes -- Determining plating efficiency -- DNA miniprep from e. coli -- Preparing and transforming competent e. coli -- Storing and handling the systematic deletion collection -- Media -- Stock preservation -- Yeast genetic and physical maps -- Templates for making streak plates -- Electrophoretic karyotypes of strains for southern blot mapping -- Strains -- Counting yeast cells with a standard hemocytometerchamber -- Tetrad scoring sheet.