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名称 Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual
索引号 Q949.32-62/E20021/2000
分类号 Q949.32-62/E20021
Q949.32(真菌门)
Q949.3(菌类)
Q949.(孢子植物-植物)
Q949(植物分类学(系统植物学))
Q94(植物学)
Q(生物科学)
作者 Burke、 Dan、 1954-、Dawson、 Dean.、Stearns、 Tim.、Cold Spring Harbor Laboratory.、
出版社 Plainview, N.Y. : Cold Spring Harbor Laboratory Press, c2000.
ISBN
页数 xvii, 205 p. : ill. ; 28 cm.
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简介
注解 Rev. ed. of: Methods in yeast genetics /Alison Adams ... [et al.]. 1997 ed. 1998. 附註 Includes bibliographical references. 內容 Looking at yeast cells -- Isolation andcharacterization of auxotropic temperature-sensitive, and UV-sensitive mutants -- Meiotic mapping -- Transformation of yeast -- Synthetic lethal mutants -- Gene replacement -- Isolation of ras2 suppressors -- Manipulating cell types -- Isolating mutants by insertional mutagenesis -- Two-hybrid protein interaction method -- High-efficiency transformation of yeast -- "Lazy bones" plasmid transformation of yeast colonies -- Yeast DNA isolations -- Yeast protein extracts -- Yeast RNA isolation -- Hdroxylamine mutagenesis of plasmid DNA -- Assay of B-galactosidase in yeast -- Plate assay for carboxypeptidase Y -- Random spore analysis -- Yeast vital stains -- Yeastimmunofluorescence -- Actin staining in fixed cells -- PCR protocol for PCR-mediated gene disruption -- Yeast colony PCR protocol -- Measuring yeast cell density by spectrophotometry -- Cell synchrony -- Chromatin immunoprecipitation -- Flow cytometry of yeast DNA -- Logarithmic growth -- EMS mutagenesis -- Tetrad dissection -- Making a tetrad dissection needle -- Pickingzygotes -- Determining plating efficiency-- Appendices.